Anti-ACTA2 Antibody, Mouse Monoclonal, A1T
產(chǎn)品編號(hào)：CM00526HuM10-A1T
$ 詢價(jià)
規(guī)格 100ul
|
產(chǎn)品名稱：Anti-ACTA2 Antibody, Mouse Monoclonal, A1T
克隆號(hào)：A1T
抗體亞型：/
經(jīng)驗(yàn)證的應(yīng)用：WB/IHC
交叉反應(yīng)：Predict reacts with: Human/Mouse/Rat
特異性：/
免疫原：A synthetic peptide of human ACTA2
制備方法：Produced in mouse immunized with ACTA2, and purified by antigen affinity chromatography.
來源：Monoclonal Mouse IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
濃度：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Application in WB
Western blot analysis of extracts of various cells lines and tissues, using ACTA2 antibody CM00526HuM10-A1T at 1μg/mL. Secondary antibody: HRP Mouse Anti-Rabbit IgG (H+L) at 1：20000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Figure2.Application in IHC
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon、human lung、monkey epididymis、monkey heart、monkey kidney、monkey liver、monkey rectum、monkey stomach、monkey tongue sections labelling ACTA2 with purified CM00526HuM10-A1T at 5μg/mL dilution. Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using citrate buffer (PH6.0). Tissue was counterstained with Hematoxylin. Mouse specific IHC polymer detection kit HRP/DAB secondary antibody was used at 1：2000 dilution. PBS instead of the primary antibody was used as the negative control.
別稱：ACT-A2, ACTSA, ACTVS, Asma, GIG46, Cell growth-inhibiting gene 46 protein
背景信息：ACTA2 (also known as 偽-smooth muscle actin or 偽-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells. This antibody may cross-react with other isoforms of actins.
全稱：Actin, aortic smooth muscle (ACTA2)