Anti-CKM Antibody, Rabbit Polyclonal
產(chǎn)品編號(hào)：PA00011HuA10
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產(chǎn)品名稱：Anti-CKM Antibody, Rabbit Polyclonal
經(jīng)驗(yàn)證的應(yīng)用：WB
交叉反應(yīng)：Predict reacts with: Human/Mouse/Rat
特異性：human CKM
免疫原：Recombinant human CKM protein, fragment Met1~Lys381; UniprotKB: P06732
制備方法：Produced in rabbits immunized with human CKM, and purified by antigen affinity chromatography.
來(lái)源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Application in WB
Western blot analysis of extracts of various tissues, using CKM antibody (PA00011HuA10) at 1 µg/mL dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Blocking buffer: 5% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.8s.
別稱：CK-MM, CKMM, CK3, CK-M, MM-CK, M-CK, Creatine kinase M-type, N-terminally processed
背景信息：Creatine Kinase MM/CKMM. Creatine kinase (CK) catalyzes the conversion of creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP). CK reaction is reversible and thus ATP can be generated from PCr and ADP (1). In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, hair cells of the inner ear, spermatozoa and smooth muscle, PCr serves as an energy reservoir for the rapid buffering and regeneration of ATP in situ, as well as for intracellular energy transport by the PCr shuttle or circuit (2). Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure. Creatine kinase M (CKM), especially, is a cytoplasmic enzyme and is an important serum marker for myocardial infarction. It acts as a homodimer (MM type) in striated muscle as well as in other tissues, and forms a heterodimer (MB type) with CKB, a brain isozyme, in the heart (3). The recombinant human CKM is in the MM type and its activity is measured using a phosphatase-coupled method (4).
全稱：Creatine kinase M-type (CKM)
經(jīng)驗(yàn)證的應(yīng)用：WB
交叉反應(yīng)：Predict reacts with: Human/Mouse/Rat
特異性：human CKM
免疫原：Recombinant human CKM protein, fragment Met1~Lys381; UniprotKB: P06732
制備方法：Produced in rabbits immunized with human CKM, and purified by antigen affinity chromatography.
來(lái)源：Polyclonal Rabbit IgG
純化：Immunogen affinity purified
緩沖液：Supplied in PBS, 50% glycerol and less than 0.02% sodium azide, PH7.4
偶聯(lián)物：Unconjugated
狀態(tài)：Liquid
運(yùn)輸方式：This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended.
儲(chǔ)存條件：This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Avoid repeated freeze-thaw cycles.
圖片：
Figure1.Application in WB
別稱：CK-MM, CKMM, CK3, CK-M, MM-CK, M-CK, Creatine kinase M-type, N-terminally processed
背景信息：Creatine Kinase MM/CKMM. Creatine kinase (CK) catalyzes the conversion of creatine to phosphocreatine (PCr) by consuming adenosine triphosphate (ATP) and generating adenosine diphosphate (ADP). CK reaction is reversible and thus ATP can be generated from PCr and ADP (1). In tissues and cells that consume ATP rapidly, especially skeletal muscle, but also brain, photoreceptor cells of the retina, hair cells of the inner ear, spermatozoa and smooth muscle, PCr serves as an energy reservoir for the rapid buffering and regeneration of ATP in situ, as well as for intracellular energy transport by the PCr shuttle or circuit (2). Clinically, creatine kinase is assayed in blood tests as a marker of myocardial infarction (heart attack), rhabdomyolysis (severe muscle breakdown), muscular dystrophy, autoimmune myositides and acute renal failure. Creatine kinase M (CKM), especially, is a cytoplasmic enzyme and is an important serum marker for myocardial infarction. It acts as a homodimer (MM type) in striated muscle as well as in other tissues, and forms a heterodimer (MB type) with CKB, a brain isozyme, in the heart (3). The recombinant human CKM is in the MM type and its activity is measured using a phosphatase-coupled method (4).
全稱：Creatine kinase M-type (CKM)